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Problems with antibodies could result in misidentification of candidates for drug development and generate a numerous misleading or irreproducible scientific data.  In general, a validated antibody shall be qualified by methods that demonstrate the following characteristics:

•Sensitivity – “on target” binding properties

•Specificity – “off target” binding properties 

•Reproducibility – produced to ensure consistency.


Thus, a validated antibody must be shown to be specific, selective, and reproducible in the contexts of its applications, diagnostically or therapeutically.  Trican Biotech. offers flexible assay portfolio for antibody validations including ELISA, Western blots, IP, IF, IHC, flow cytometry and related performance assay, such as cell-based analysis.

Assays and approaches for antibody validation

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