TRANSIENT PROTEIN EXPRESSION

Transient protein expression provides early access to protein of interest or antibody expressed in a mammalian system without the lengthy developmental time needed for developing stable cell line.  Trican Biotech. offers fast and high quality services for recombinant antibody expression services (soluble Fab, scFv, full IgG, bi-functional, or fusion protein) using mammalian cells in small/large scale transient transfection.  In addition to recombinant antibody production, Trican’s transient expression system can also be used to prepare secretory protein/antigen for various applications.  Our expression platform supports native post-translational modifications and proven to be able to retain natural conformation of antigens/proteins, and biologics.  Chinese Hamster Ovary (CHO) and Human Embryonic Kidney 293 (HEK) are the main primary cell lines used for transient expression due to the ease of handling, robust growth rates, excellent transfectability and high yield recombinant protein-generation capacity.

Transient Antibody Production Workflow